I want to estimate CD-4 and CD-8 in the spleen, please suggest an affordable kit for magnetic bead method estimation without using flow cytometry.
pl tell the specie if mice or rat bd bioscience is best
First you will have to get total T cells and then you may use either BD biosciences or R&D system kits for CD4/CD8 cell isolation kits based on magnetic bead.
make sure you use kit is specific to animal you are using
Good luck
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