I want to examine the allelopathy (through root exudation) of two species to determine and compare the relative allelopathic potential of both species.
Prof Ray Callaway produced a series of experiments in presence or absence of activated carbon, AC, that allow to test allelopathic combinations in a greenhouse on potted plants. If AC alleviates growth competition in pots with two species planted togheter there is a way of extracting active substances that were absorbed on AC beads. To extend this set to duality you might use a third species as passive control, but generic lists of references are in http://5e.plantphys.net/article.php?id=377
Allelopathetic studies are complex in nature. At the moment no study on this discipline have been conducted at Njala University, due mainly to lack of laboratory to determine the allelopathetic compounds. Information so far has been obtained from literature.
It is important to note that all plants are allelopathetic, though the level may defer from one plant species to another.
We also work on allelopathic root exsudates. We recently published the method we are working with (Falquet B, Roux D, Henriet L, Tschuy F & Wirth J (2014) Simple method to separate resource competition from allelopathic root interactions. Allelopathy Journal 34, 227-240.) Recently we have added a permeable membrane to the experimental setup in order to see whether allelochemicals diffuse in the soil solution. I would be interested to discuss your research work with you. If you are interested, please contact me by e-mail.
Different methods are available for such purpose. Equal compartment method, double pot method, dose dependent studies, just to name a few. We have been working on screening of different plant materials for their allelopathic studies. I will recommend you to contact Professor Leslie Weston at CSU, Min An and Hen Wen Wu. I am interested in your studies and the methods you are using for your studies. You may contact me by email.
Take a look at
Mendes ID, Rezende MOO (2014) Assessment of the allelopathic effect of leaf and seed extracts of Canavalia ensiformis as postemergent bioherbicides: A green alternative for sustainable agriculture. Journal of Environmental Science and Health Part B – Pesticides Food Contaminants and Agricultural Wastes 49: 374-380.
You might want to look at "Population Biology Of Plants" JL Harper.
Chapter 11 Mechanisms of interaction between species. There is a nice discussion on this topic and there are some good references to follow.
Good question. In my work this is observed that LantanaLantana camara inhibit the growth of Parthenium hysterophorous and also stimulate it.It is due to allelopathy.
You can view my different articles on RG.
Data Allelopathic effect of cassia tora extract....
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