How do I analyse a Photoluminescence Spectrum ?

I have a few questions related to PL (images attached for same material by 2 methods), It would be great to get some help. 1. What is the physical meaning of highly intense PL? Does this mean...

02 February 2021 1,791 3 View

How to choose the electrolyte for (electrochemical impedance spectroscopy) EIS ?

Hi, I am working on a multi cationic material that has Li in it. I wish to measure the conductivity of my sample. We have a 3 electrode system with Ag/AgCl as a ref, platinum, and glassy carbon...

29 January 2021 405 2 View

Rhodanese enzyme assay using salt potassium ferricyanide?

I wanted to perform rhodanese enzyme assay using salt potassium ferricyanide (K3Fe(CN)6). The assay is based on the products formed in the reaction but I can't get the reaction i.e what product...

26 January 2021 8,044 4 View

How to get rid of a constant peak at fix RT every time in HPLC ?

I am running catecholamine standards in HPLC using UV/FLD detector. I am getting a constant peak at fix RT (retention time @ 3.5 min, image enclosed) every time, even if I run another standard or...

29 September 2020 847 7 View

Is there any design method for control system without linearization of nonlinear plant?

Usually, a nonlinear plant needs to be linearized before using some design method for control law synthesis. Is there any design method, which does not require a linearization?

14 September 2020 1,268 2 View

LC3 II form remains unchanged but p62 protein is increasing, why?

Hello, I am treating my cells with a compound to study whether the autophagy can be affected or not. Using Western blot, I found that LC3 II form remains unchanged but p62 protein is increasing ....

16 August 2020 8,937 3 View

How do I get the permission for using the images of a published article?

How do I get the permission for using the images of a published article?

03 August 2020 9,083 3 View

How do I model a protein with length of more than 2000 amino acid for MD simulation using I-Tasser?

I am using I-TASSER to model a protein of more than 2000 amino acids for MD simulation but the highest limit of the sequence in I-TASSER is 1500 amino acids. How do I solve this problem? One way...

10 June 2020 1,168 3 View

Nonspecific PCR products and faint bands with Q5 polymerase (0for overlapping PCR)?

I am currently using Q5 polymerase from NEB to amplify a protein of 2.5 Kb. I have designed the primers to target the 21 BP that are compliment to the DNA sequence , the restriction sites are...

30 April 2019 9,707 3 View

How do we analyse photo luminescence spectrum of ZnO?

I have performed PL for my ZnO sample synthesized by hydrothermal method using NaOH and HMT. The PL emission was recorded at excitation wavelength of 320 and 400nm. Both the obtained emissions are...

03 January 2019 4,247 3 View

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02 March 2021 3,060 3 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View