Hi Tahira,

In our experiments, SNU-251 cells purchased from the American Type Culture Collection, were cultured in RMPI 1640 medium supplemented with 10% FBS and antibiotics at 37°C in a 5% CO2 incubator. So, your medium content looks OK.

Best wishes,

Ilya

Dear sir 

You can use DMEM high glucose including L-glutamine and Sodium pyruvate, i think this the best

Serag Eldin   

hi Serag. Can u provide me the reference for using this media ? i have not seen this media in contents. 

Thanks.