I want to express a mammalian protein (179 amino acids) in CHO cell line. please suggest me any vector with HIS tag and GFP tag (selection purposes) which will suitable for CHO cells.
The pET vectors were always my go-to for mammalian cloning vectors, I've used pET-15b and pET-28a but they don't have GFP. Addgene has pET vectors with TEV-cleavage site and GFP as a fusion or GFP co-expressed with IRES.
Vincent Paul Schmitz sir, its for bacterial expression and I want to express my protein in mammalian cells (CHO cell line). Do you have any exposure with mammalian vector?
Subhash Ph.D. - Ah, I misread, in that case you have a few options. The TetOn is great if you want a dose-response inducible system (6xTetOn), the CMV is a fantastic pre/promoter, as is SV40 & Puro. It depends on what restriction sites you require in your MCS for gene insertion, whether you intend to do a transient or inducible expression, and if you would like plasmids that are Lentiviral-ready for retroviral transduction.