I want to study ligand-protein interaction using 3 dimensional fluorescence spectroscopic measurements. Please guide me as to how to set parameters and perform the study in the software.I am using a Carey Eclipse Fluorescence Spectrophotometer.
Thank you for the suggestion
Hello, I am interested in understanding the relationship between binding energy (B.E.) and ions' oxidation state (O.S.) in XPS spectra. Per example The core-level XPS of Mn2P in MnxNy can be...
21 December 2022 1,710 2 View
I have done a polymerization using toluene as the solvent, when I dried the precipitate it changes to black and sticky. can anyone suggest me a technique or any other method to get my sample as solid?
15 February 2022 2,689 5 View
Do we have to use both the ROX dye and SYBR while performing qPCR analysis?
08 November 2021 4,139 3 View
Hello! Hope you are doing good. I am a post graduate student from India, who has undertaken a research as part of my curriculum. My thesis research is about K-Pop fans and how being part of a...
12 September 2021 2,485 0 View
Initially the motor coupled to load with flexible coupling drive shows oscillations in shaft speed at both sides of the coupling . The flywheel at the non driving end of the motor is installed...
06 October 2020 3,190 2 View
D and G band in CARBON DOTS observed at 1304 and 1441 cm-1 unlike 1348 and 1581cm-1. Can someone help me in knowing the reason behind this shift. I am attaching recorded raman spectra of carbon...
05 July 2020 8,401 0 View
I have used 300 oC as degassing temperature for BET analysis of clay nanoparticles, it got charred after degassing. Why does this occur?
06 June 2019 987 5 View
XRD peak of bare carbon dots coming at 19 degrees and surface functionalised carbon dots with hexadecylamine coming at 19 degrees and 20-22 degrees, can someone help me in analysing these peaks...
25 March 2019 1,265 5 View
I see circular rings in HRTEM image of carbon nano particles, Can someone tell me what does it represent?
18 March 2019 8,235 3 View
1.I want to know how to get Thai character numbers(0-9) dataset and train them and which deep learning algorithm is more efficient.
19 July 2018 2,399 2 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
How to calculate the molar proportions of the oxides from the XRF analyses?
02 August 2024 2,565 2 View
Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.
31 July 2024 9,140 2 View
Hello everyone, I am new to this characterization but watching videos on youtube I have already plotted a Nyquist plot (Z' and -Z'') and already fitted models on my material using Zsim. But now I...
29 July 2024 1,376 0 View
While I am doing HER, I noticed that nyquist plots of Electrochemical Impedance Spectroscopy (EIS) is not complete. Will it affect for the Linear Sweep Voltametry (LSV) data? However, I can found...
26 July 2024 7,542 4 View
In the context of Electron Spin Resonance (ESR) spectroscopy, under what conditions is an absorption spectrum considered to be a single absorption spectrum?
25 July 2024 2,325 1 View