I have been performing quantification using crystal violet assay but the results are not reliable as it is not reproducible,is there any other relaible method for quantification of biofilms ?
https://www.researchgate.net/publication/5693013_Peeters_E._Nelis_H._J.__Coenye_T._Comparison_of_multiple_methods_for_quantification_of_microbial_biofilms_grown_in_microtiter_plates._J._Microbiol._Methods_72_157-165
Article Comparison of multiple methods for quantification of microbi...
CLSM or Tissue culture plate method followed by OD reading is better, I think..
I want to know more about Uranium ore deposits in world.
11 August 2024 6,720 0 View
I want to know more about diamond ore deposits in world.
11 August 2024 2,167 1 View
We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...
10 August 2024 6,678 14 View
If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?
10 August 2024 8,198 5 View
I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...
10 August 2024 3,229 0 View
What are a “Farmers Producer Organization” (FPO) and its essential features?
10 August 2024 477 5 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...
08 August 2024 4,831 4 View
How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?
08 August 2024 8,162 0 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
My colleague and I started research project about bioplastic made out of starch-curcumin composite. However, we are currently having trouble regarding the produced film. We have degassed the...
01 July 2024 9,419 0 View
Hi all, Would anyone have any suggestions for articles and protocols for performing microdilution tests on candida biofilm? Do you recommend diluting the drug in the medium and adding it to the...
19 June 2024 4,420 3 View
Is there scientific evidence that proves that the presence of N-acyl homoserine lactone can account for the bacterial biofilm population?..
13 June 2024 3,776 2 View
biofilm
31 May 2024 1,969 4 View
I am preparing a protocol for SEM of a biofilm, but do not have easy access to 100% ethanol for the dehydration steps. Can 100% ethanol be substituted for 100% alcohol (95% EtOH, 5%...
07 May 2024 1,515 3 View
I need some clarification about metaproteomics. In the wastewater (or) biofilm samples, I plan to find out which specific proteins and enzymes are causing the severe corrosion on the metal surface...
21 April 2024 5,315 2 View
Lactobacillus planetarium and Pediococcus pentosaceus against foodborne pathogens.
18 April 2024 8,127 0 View
Based on your expertise, which softwares are the best for the data analysis and graphs for the quantitative study of microbial biofilms?, pros & cons?
08 April 2024 8,304 1 View
I use BG 11+ Media but with an alternative trace metal mix.
17 March 2024 9,253 3 View