Abhishek Dubey

4 Questions 4 Answers 0 Followers

Questions related from Abhishek Dubey

What decides if alcohol dehydrogenase is NAD+ dependent or NADP+ dependent ?

what decides if alcohol dehydrogenase is bound to NADH or NADPH ? some enzymes contain NADPH as bound co-factor but does not show invitro dependency to NADP+, instead are active with NAD+ ?

01 September 2017 9,765 1 View

Rescue Cloning of Bacterial Genomic DNA using the EZ::TN™ <R6Kγori /KAN-2>Tnp Transposome ?

Hello everyone,I have been trying rescue cloning experiments as per the protocol suggested...

28 September 2015 5,964 5 View

Can anyone suggest me a method for quantification of M.smegmatis Biofilm ?

I have been performing quantification using crystal violet assay but the results are not reliable as it is not reproducible,is there any other relaible method for quantification of biofilms ?

15 September 2015 4,594 2 View

Can someone help me with media and protocol for M.smegmatis biofilm setup?

I have been trying to setup M.smegmatis biofilm with both sauton's and M63 minimal media but not getting the desired morphology as the one reported in literature.

22 April 2015 7,920 2 View