Can anyone suggest me a good source of cultures representing different toxin types of clostridium perfringens?

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PC Vs KL mode of analysis in Diversilab system by biomerieux?

There are two modes of analyzing DNA fingerprints by Diversilab system.According to the analysis guide,while switching from PC to KL mode the percentage of average similarity should increase....

09 October 2016 8,079 1 View

Why does Clostridium perfringens not produce black colonies in TSI slant ?

I have been making TSI slant from clostridium perfringes biochemical tests.But has per literature I could not find any Black precipitation.Where as media turned yellow due to acid production.

02 March 2015 1,258 16 View

Any suggestions on Real time PCR Vs CFU count?

I am doing a quantification experiment for c.perfringens from fecal samples.I am following a paper which describes that DNA was isolated from the stool sample by Qiagen stool kit.After that they...

02 March 2015 5,760 3 View

Non specific peaks in real time PCR?

While performing real time assay I have noticed that peaks arises at the very beginning. It is not for any dimer as the NTC does not show any peak. These initial peaks are very inconsistent and...

09 October 2013 3,332 1 View

How to design primers specific for RT-PCR in case of bacteria ?

To avoid amplification of genomic DNA.

03 April 2013 8,189 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View