Md Iftikar Hussain

6 Questions 19 Answers 0 Followers

Questions related from Md Iftikar Hussain

PC Vs KL mode of analysis in Diversilab system by biomerieux?

There are two modes of analyzing DNA fingerprints by Diversilab system.According to the analysis guide,while switching from PC to KL mode the percentage of average similarity should increase....

10 October 2016 8,045 1 View

Any suggestions on Real time PCR Vs CFU count?

I am doing a quantification experiment for c.perfringens from fecal samples.I am following a paper which describes that DNA was isolated from the stool sample by Qiagen stool kit.After that they...

03 March 2015 5,726 3 View

Why does Clostridium perfringens not produce black colonies in TSI slant ?

I have been making TSI slant from clostridium perfringes biochemical tests.But has per literature I could not find any Black precipitation.Where as media turned yellow due to acid production.

03 March 2015 1,223 16 View

Non specific peaks in real time PCR?

While performing real time assay I have noticed that peaks arises at the very beginning. It is not for any dimer as the NTC does not show any peak. These initial peaks are very inconsistent and...

10 October 2013 3,290 1 View

Can anyone suggest me a good source of cultures representing different toxin types of clostridium perfringens?

ATCC cultures are not available outside USA except one. Even extracted DNA will work for me.

08 August 2013 9,425 2 View

How to design primers specific for RT-PCR in case of bacteria ?

To avoid amplification of genomic DNA.

04 April 2013 8,143 2 View