I need to detect both the cellular body and the axon in rat brain slice.
Hi Francesca,
MAP2 is a very good marker for highlighting neuronal body, at least in pyramidal cells.
Hope this helps,
Ezequiel
Thank you all for the answers!
I'm currently using NeuN for soma detection and I'm looking for a good axon staining: other of my group used B-tubulin III with poor results. I'll try to use also the MAP-2 and maybe to improve the B-tubulin III stain protocol.
Hi Francesca,
For soma and generic dendrite staining, I suggest you may use Neu-N and Tuj1 or MAP2 respectively.
However, If you would like to specifically characterize the axonal extensions, you may try anti Tau-1 antibody which has high clonal specificity to growing axons. Ofcourse B-tubulin III(i.e Tuj1) can stain the axons, but with lot many other dendrites which may seem bit messy in high density cultures.
Hence you may select species reactivity of Anti-NeuN and Anti-Tau1 and use them in conjunction for Soma + Axon staining.
Supplier: Abcam: Anti-Tau antibody (code: ab64193)
Cheers!
Shreyas
Hi Francesca,
MAP2 is usually a better marker for dendrites, neurofilament is a good marker for axons (or SMI-32, the unphosphorylated form is good for outlining axons in the grey matter). We use a combination of Neun, MAP2 and neurofilament (chicken one from millipore is very good) that together will label the vast majority of the cell.
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