Can anyone suggest how to prepare a buffer to extract proteins from soybean seeds?
I'm thinking about to use the extraction buffer (50% [v/v] phenol, 0.45 M Suc, 5 mM EDTA, 0.2% [v/v] 2-mercaptoethanol, 50 mM Tris-HCl, pH 8.8). Can anyone recommend it? How to get rid of abundant proteins?
If you have difficulty in this process, I suggest to you to determine N through digestion then multiply x a factor to get protein% . Wish you luck.
Please check it.
Article Proteins of Soybean Seeds: I. Isolation and Characterization...
Greetings and Regards I did not work on this, I ask my colleagues, if I find a case, I will send it to you.
Dr. Seyed Mohsen Hesamzadeh Hejazi from the Research Institute of Forests and Rangelands Email them and they will guide you.
Good luck
Here is a worthy reference for extracting low abundance proteins from soybean seeds.
An efficient extraction method to enhance the analysis of low abundant proteins from soybean seed - ScienceDirect
For selecting the protein solubilization method best suited to your needs, you may read this reference.
Comparison of protein solubilization methods suitable for proteomic analysis of soybean seed proteins - ScienceDirect
I wish you to succeed in your R&D work.
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