Hello Shivam

There are companies like Abcam, R&D Systems, Life Technologies, Novus Biologicals where you can purchase lyophilized VEGF protein, reconstitute and use for you experiments. 

Agree. Biocompare is a good website for comparing the product and pricing from different companies and selecting the right one for your experiments.

I would add: don't forget to add 0.1% BSA to regain full activity of your VEGF!

It's important to check which VEGF isoforms you want to use, though the most commonly used is VEGF165. VEGF121, 189, 164 etc are also available from R&D depending on the question you want to ask. Another thing is check the source of protein. Production from E.coli can sometimes lack post translation modification of VEGF that one might find in mammalian cells. So, given the choice, I would suggest recombinant protein produced in HEK cells. 

hello shivam,

  If you have to initiate a small trial expt. you could take some microliter from my lab, when u visit lucknow.

KJ