Can anyone suggest how to avoid retention time shift in biogenic amine estimation using a UV-VIS detector?

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How to obtain slope distribution of an AFM scan using Nanoscope?

I have AFM scans of a uniformly textured surface of 16x16um size. I would like to obtain the slope distribution of the surface using Nanoscope, as I plan on trying to relate this parameter to the...

10 April 2024 5,024 2 View

Suggest any good article on Gel Propellants used in aerospace applications. Also are gel propellants currently in use?

gel propellant, rheology, aerospace

23 January 2024 9,151 1 View

Can anyone suggest a free citation manager alternative to Mendeley?

Over the last 10 years, I have been using the Mendeley citation manager. Due to their new policy, it is not working as smoothly with MS Word. Therefore, I am looking for a good alternative.

10 January 2024 3,460 5 View

Is the guide RNA (gRNA) designed for gene knockout has possibility of binding to messenger RNA (mRNA) of the target gene?

In CRISPR/Cas, we design guide to make double strand break in a specific site of the genomic DNA. I am curious to know, whether the gRNA-Cas9 complex has any possibility to bind to the messenger...

25 December 2023 630 1 View

If I want to knock out an essential gene from E.coli genome and replace it with a homologous gene from another genus. What should be the strategy?

If I want to knock out an essential gene from E.coli genome and replace it with a homologous gene from another genus. What should be the strategy I Should follow

25 October 2023 3,773 2 View

How to define predefined field in Friction Stir welding simulation using CEL Method?

I am trying to simulate FSW in CEL using abaqus. I am trying to replicate exactly the same simulation of this paper :Coupled Eulerian Lagrangian finite element modeling of frict... . But the...

04 August 2023 3,782 0 View

How to simulate evaporation through porous media with evaporation-condensation method in ansys fluent?

in the model I am simulating, the mixture of ethylene glycol and water is flowing in the hollow fiber membrane (a cylindrical hollow tube made up of porous media). during the flow, the water in...

19 July 2023 7,116 1 View

How to couple thermal boundary conditions such as covection in SPH method in Ansys LS-Dyna?

I have been trying to simulate the Friction Stir Welding process in Ansys LS-Dyna using the SPH method. I am struggling to couple it thermally to find the temperature, add convection, etc. Please...

12 July 2023 8,181 0 View

How to resolve this error '***ERROR: Excessive rotation of node number TOOL-1.81743' ?

I am trying to simulate Friction Stir welding using the CEL method. I have resolved all other errors except this particular error. Someone kindly help me with this. I have attached the CAE file.

11 July 2023 8,959 2 View

Sanger sequencing giving multiple peaks after few base pairs. What are the possible reasons? How can I confirm that? Suggest me way to get the seq ?

I am analyzing the gene sequence of my target gene in Brassica juncea. In that there is some ambiguities in the gene sequence which is appearing after few clear peaks in the sequence. I am...

27 June 2023 9,927 1 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

How can I add own Henry coefficients in Aspen Plus?

Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?

05 August 2024 2,333 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

Can a Conventional 50W visible LED Lamp Emit UV Light?

I'm researching light sources and their UV emissions. I'm curious if a 50W, 7500K conventional LED lamp emits any UV light. I found that a 200W tungsten lamp can emit up to 1.5 mW/cm² at 350 nm....

31 July 2024 3,843 7 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View