i want to start molecular diagnosis unit in my lab. so i need highly specific kits for different genetic disease. I don't have Real-Time PCR, I just have conventional gradient PCR machine, so i need that kits only which can work with it. For Qualitative analysis ( Diagnosis) only.
Jigarkumar Suthar
I need only qualitative analysis kits, because i don't have RT PCR.
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Marco flavio michele Vismara
In a basic setting, reverse dot blot technique can be useful: machines are quite inexpensive and allows to make optimal diagnosis without an rtpcr machine or a sequencer. There are excellent kits and you need just a pcr machine (autoblot machine is optional, can be done free hand).
Hope it helps.
https://en.m.wikipedia.org/wiki/Dot_blot
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