Ranjith Kumar Manoharan

7 Questions 14 Answers 0 Followers

Questions related from Ranjith Kumar Manoharan

Can anyone suggest better protocol for extraction of yeast RNA?

I am dealing with Candida RNA isolation from TRI reagent (used qiazen kit for extraction).I am really concerned about the quality of my RNA. According to Nanodrop reading my yields were ~100 to...

04 October 2016 8,316 3 View

Hi, can someone please suggest guidelines for measuring C.albicans biofilm for crystal violet staining method?

I'm dealing with C. albicans biofilm assay in 96 well plates. However, I did not get good result after washing with water and CV staining. I could not see any cells after initial water washing. C....

18 May 2016 5,683 8 View

Can anyone suggest the optimal Salt Adjusted temperature for PCR primers?

I'm using (http://www.basic.northwestern.edu/biotools/oligocalc.html) online tool for primer designing. What would be the optimal salt adjusted temperature for PCR primers?

21 October 2015 6,210 3 View

How can I get p value using FPKM in RNA seq data?

I'm dealing with RNA seq data and obtained FPKM value using cufflinks for treated and control samples. Do i want to normalize these value to obtain p-value or it is sufficient to find DE genes?....

14 September 2015 8,099 6 View

How can we isolate specific fungal DNA from soil?

I am using kit for direct isolation of DNA from soil samples. However, I am unclear whether the origin of these DNA sequences are expected to be fungal or not, since primers were from ITS regions.

30 October 2014 2,412 4 View

Can anyone recommend a fungal DNA extraction protocol from soil samples?

Maybe a kit or modified method?

17 October 2014 9,861 6 View

Which tool is best to find SNPs from a minimum number of mRNA sequences(10)?

I dont't have a linux system, so please suggest a windows based tool. I have tried with some of the online tools like...

28 April 2014 5,212 0 View