14 Questions 16 Answers 0 Followers
Questions related from Najmin Ahmed
I am preparing sample for SEM analysis, but lacking the facility of liquid.co2 and HMDS. So, is there any other method to dry the sample ? Your suggestions will be highly appreciated .
14 December 2022 6,644 6 View
Performing scan electron microscopy for gill tissue and i am looking for if i can keep it for overnight or not in 2.5% glutaraldehyde.
28 November 2022 8,905 1 View
Can we store a prepared sample in drying stage (in Liq. Nitrogen) for 1 week after completion of fixation and dehydration, before mounting on a stub ?
28 November 2022 2,664 3 View
am running sds page and now based on the molecular weight of resulted bands i need to identify the possible enzymes or protein present in my sample. So, is there any database, by which i can...
28 October 2022 472 3 View
I am.performing LCMS analysis of my target sample (crude) for its proteomic analysis and After LC-MS analysis, i would need to check for properties and function of target proteins or enzymes...
24 September 2022 7,060 4 View
Hello, i am sending protein sample for LC-MS analysis for the first time, can you suggest how to proper pack it to maintain a 4 degree Celsius medium. Two choices have been given either DRY ICE or...
23 August 2022 1,768 4 View
I have a vertebrate tissue sample and i need to seperate each enzyme present in the sample by performing PAGE I need your suggestion in this regard, which gel run should i perform ? Is it NATIVE...
10 June 2022 4,260 0 View
I am preparing a gill tissue for SEM image, and its size is approx 1.5 × 1 m.m, and i have formaldehyde. Can i fix my sample in formaldehyde ? Can i skip osmium tetroxide use ? For SEM analysis
10 December 2021 1,745 2 View
Can it be stored ? If it is then in freezer or at room temperature ?
27 October 2021 4,304 9 View
I am isolating whole protein from a fish tissue, now i want to know, using protease inhibitor is it must ? Or can proceed using PMSF only ?
04 October 2021 4,590 3 View
I am working on protein isolation by using RIPA buffer, the composition which I am using is (50 mM TrisHCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.5% Sodium deoxycholate, 0.1% SDS, 1 mM EDTA),...
22 April 2021 4,792 3 View
Atmospheric oxygen amount is around 21 mg/l and when we talk about dissolved oxygen in water it is around 15mg/l at 0 degree Celcius and 6-8 mg /l at 25 degree Celcius. My question is how fish...
12 March 2021 7,828 4 View
I am looking for most preferable ways of drying plant material for its extract preparation. Whether to use shade dry, freeze dry, oven dry or to go with another method; suggest me effective way...
22 December 2020 6,614 6 View
How polymerase reads ori site where it is already methylated in GATC sequence ?
22 March 2020 8,972 5 View
