We have been testing reducing strength at root surface using DCPIP and potassium ferricyanide (as shown in our recent publications that appeared in PLoS One, Biometals and RSC Advances). I am looking for a protocol that is superior to this method.
Hi, I'm not too sure if this would work better than your current method, but I thought I would suggest it. I would suggest performing the experiment in an electrochemical cell to detect reduced DCPIP or ferricyanide. In this way you can get a real time monitoring of the reduction of these compounds by the root.
Thanks Michael. It is a good idea. We did make an attempt in this direction with my colleague in Chemistry, but failed. We felt that spectrophotometric method to detect reduced and oxidized states of DCPIP and ferricyanide is better (as their reduced & oxidized states have very specific and distinct absorption maxima).
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