14 August 2021 1 10K Report

I am heterologously expressing a G alpha protein along with GST tag in BL21. Can any one suggest a protocol to purify the active form of the protein which could be used for further experiments?

More Shilpa Sarkar's questions See All
How to get links for copyrights for papers?

how to get links for copyrights for papers?

06 August 2024 7,410 1 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

How to perform band structure calculation of a bulk, nonmagnetic superconductor with spin-orbit coupling using vasp?

I'm new to VASP and I'm trying to calculate the band structure of a bulk, nonmagnetic superconductor with a tetragonal crystal structure. I'm including spin-orbit coupling (SOC) in my...

09 July 2024 5,641 3 View

Do social development programs integrate women into climate change adaptation initiatives, especially within the framework of poverty reduction?

Gender, along with socio-economic and environmental factors, is a key determinant of vulnerability to climate change.

24 June 2024 8,437 2 View

Why the number of atoms is different in xyz file and POSCAR file?

am trying to make POSCAR file and xyz file using vasp for NbReSi compound using VESTA. However, I notice that the number of atoms for xyz file is given as 41 whereas for POSCAR is it 36. Can...

17 April 2024 698 2 View

Alternative to nitrogenase?

I have seen nitrogen fixation in three heterotrophs, but cannot detect any nitrogenase in their genomes. Are there any alternative pathways that I can look into that may provide information about...

01 April 2024 5,641 0 View

XRD peak deviation?

In XRD analysis, when we move to a higher angle, like above 50 degrees, if I have two reference lines of different phases, one matches the peak and the other is towards the right of the peak with...

31 March 2024 1,812 2 View

What would be a standard protocol for termite sampling in sub-tropical Himalayan foothill forests?

My study area is in eastern Himalayan foothill landscape which is a tropical to sub-tropical forest; elevation ranges from 150 to 1300 meter. The forest is some parts dominated by sal, tick and...

21 March 2024 8,297 1 View

Help regarding Geometry Optimization of a bimetallic surface?

I am trying to perform a geometry optimization (GEO_OPT) of a FCC 111 Pt_{0.5}Au_{0.5} bimetallic surface (10x10x3) generated from ASE, with a lattice parameter of 3.9 A∘A∘ in CP2K. However, after...

18 March 2024 3,328 2 View

What is the effect of autoclaving PDA media twice consecutively? (without remelting, immediately after first autoclaving)?

What happens if the media is double autoclaved immediately after the first autoclaving? I am going to inoculate the Trichoderma; will it affect the sporulation of the Trichoderma?

25 February 2024 3,061 0 View

Similar questions and discussions
Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

What do you consider to be the most relevant elements of EEG for studying cognitive biases?

I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...

31 July 2024 7,259 1 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

Ti6Al4V - Phase differentiation between alpha and alpha prime martensite?

Dear Researchers, Is it feasible to distinguish between alpha and alpha prime martensite in the Ti6Al4V alloy under rapid solidification in the as-cast state using XRD, in addition to...

30 July 2024 3,849 0 View

Why only alpha wave of brain are found in some patients?

I have found an EEG where only alpha waves are present. Beta waves are not found in active patients. What interpretations ?

26 July 2024 4,741 1 View

Convergence criteria not met but ends with normal termination. Could not open the log file in gaussview. How to rectify this problem?

>>>>>>>>>> Convergence criterion not met. SCF Done: E(RB3LYP) = -472.437545326 A.U. after 33 cycles NFock= 32 Conv=0.29D-07 -V/T= 2.2914...

16 July 2024 8,862 2 View

Looking for a bioinformatics expert especially one who has expertise in Nanoparticles docking ?

Silver Nanoparticles docking with alpha amylase and alpha glusidase .

11 July 2024 6,883 2 View

ATG in pPICZalfphaA vector: Yes or Not in front of the gene of interest ... ?

Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...

10 July 2024 3,050 5 View

How do I make 10 cc of enzyme solution with a concentration of 0.2 units/mL?

Hi We have alpha-amylase enzyme that written 5 units /mg protein on its container. How do I make 10 cc of enzyme solution with a concentration of 0.2 units/mL?

08 July 2024 2,285 2 View

Can I use two different ranges of 5-point Likert scale (0-4 and 1-5; adapted from two different authors) in my questionnaire?

Good day everyone! I have searched and read a lot of topics about this one and yet there are still varying answers left and right. I need a specific one so I hope someone can help me with this. I...

02 July 2024 1,772 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close