Can anyone suggest a protocol for making Bortezomib-resistant U266/RPMI8226 cell lines?

15 July 2014 0 1K Report

I am trying to make Bortezomib-resistant U266/RPMI 8226 cell lines. I was reading few protocols but can not really make out which one is the best. Can anyone please suggest a suitable protocol?

Badges
Science topic

Similar topics
Filing

More Prahlad Raninga's questions See All

What is the procedure to lyse Gibberella Zeae spores/ mycelium?

I am looking for a membrane bound enzyme, hence wanting to separate the cytosol and cell wall of Gibberella Zeae ? Any procedure to do the same? Any help and advise is appreciated. Thanks in advance.

26 May 2020 1,946 0 View

What can be used as a ROS scavenger in vitro to eleminate intracellular ROS?

Apart from NAC, which ROS scavenger and in which concentration can be used to remove intracellular ROS generated in response to any given treatment in cancer cells to analyze the ROS dependance?

10 February 2015 1,414 11 View

Can anyone suggest a way to detect Bcl-xL, cIAP-1, and survivin in MM cell lines, RPMI 8226 and U266?

I am unable to detect Bcl-xL, survivin, and cIAP-1 protein levels in RPMI8226 and U266 cells. I used Santa Cruz antibodies in 1 in 1000 dilution. I treated cells with a drug for 24 hours, prepared...

19 January 2015 9,455 6 View

Protocol for Detection of Reactive oxygen species (ROS) using CM-H2DCFDA in THP-1 cells?

09 May 2013 2,412 3 View

Creating a simple 5G network topology in NetSim.

How can I create a topology having one BS with multiple antennas and multiple UE with multiple antennas each? I would also like to know to fix the number DL and UL layers? Can anybody suggest a...

01 January 1970 9,298 10 View

NetSim simulation - Configuring Random packet sizes and inter-arrival times

How can I configure random packet size and random packet arrivals for data traffic in NetSim?

01 January 1970 8,606 3 View

How do I create a customized project for VANET in NetSim?

What files should I include to write my own algorithms? Once that is done, how do I interface with SUMO?

01 January 1970 3,904 8 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

What should Berlin do as a city to become as impactful as London and Paris in World Football?

Please go through my Abstract. I can also share a proposed Thesis Outline.

04 August 2024 2,077 0 View