Prahlad Raninga

3 Questions 5 Answers 0 Followers

Questions related from Prahlad Raninga

What can be used as a ROS scavenger in vitro to eleminate intracellular ROS?

Apart from NAC, which ROS scavenger and in which concentration can be used to remove intracellular ROS generated in response to any given treatment in cancer cells to analyze the ROS dependance?

11 February 2015 1,379 11 View

Can anyone suggest a way to detect Bcl-xL, cIAP-1, and survivin in MM cell lines, RPMI 8226 and U266?

I am unable to detect Bcl-xL, survivin, and cIAP-1 protein levels in RPMI8226 and U266 cells. I used Santa Cruz antibodies in 1 in 1000 dilution. I treated cells with a drug for 24 hours, prepared...

20 January 2015 9,426 6 View

Can anyone suggest a protocol for making Bortezomib-resistant U266/RPMI8226 cell lines?

I am trying to make Bortezomib-resistant U266/RPMI 8226 cell lines. I was reading few protocols but can not really make out which one is the best. Can anyone please suggest a suitable protocol?

15 July 2014 1,303 0 View