I am looking at the changes that occur in the endometrium before and after uterine artery embolisation (UAE) in women who have uterine fibroids.
UAE will cause hypoxia to the uterine tissue and the fibroid tissue as soon as it is performed, however, only the fibroids will undergo cellular necrosis that is identified by MRI. I am looking for an immunocytochemistry marker that can detect chronic hypoxia in the endometrial samples. My samples are 6 months apart one prior to UAE and one 6 months after UAE.
You may wish to look at this article DOI: 10.1016/S0002-9440(10)64038-2
Would Hypoxyprobe http://www.hypoxyprobe.com/ meet your requirements?
You can look at VEGF,EGFR, Cox2 CA-9 and apoptotic markers should increase.
Thank you for your replies. My tissue is human endometrium and my samples are before and 6 months post uterine artery embolisation (UAE) .
UAE causes hypoxia initially to the different layers of the uterus and fibroids. However 6 months down the uterus functions well.
Would hypoxyprobe , EGFR and CA-9 be able to demonstrate through histochemistry staining that the 2nd sample of endometrium ( 6 months post UAE) has undergone chronic hypoxia and therefore proves that it isn't functioning as well as it was prior to UAE ?
VEGF and Cox2 : I will be using them.
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