Why Negative peak eluted in HPLC analysis?

I'facing problem while doing HPLC analysis due to negative peak was eluted which was corresponding to the principle peak RT. I tried to remove, with different solvents, but no use. i want to know...

26 November 2020 1,960 5 View

Can anyone please tell how to define link for Tuned mass Dampers using Sap2000/Etabs?

Dear Research scholars, I was doing my MTech thesis on pendulum based TMD and I was having trouble defining the link properties. I even followed the tutorials put on CSI website. still, I wasn't...

11 November 2020 8,309 11 View

How to solve "cz freeze python error in main script" Acp in Ansys probelm?

Greetings, I'm currently using the ANSYS 2020R1 crack version. I used this software for at least one month smoothly. But in the last few days, I'm facing this problem. I'm tired to solve this...

09 November 2020 6,431 2 View

What is the suitable optical filter for filtering 120MHz spacing combs from mode-locked laser for f-2f interferometry?

how to do f-2f interferometry from an octave spanning supercontinnum with frequency spacing 120MHz. Which would be the most suitable optical filter in this range for filtering f and 2f components...

12 October 2020 4,470 1 View

How we can design hybrid system between deep learning and swarm intelligence system or deep learning with fuzz?

Hybride intelligent system is good techniques used for solving diffrent optomization problems. Like classification, recognition ....etc Deep learning can be used with and hybrid with other machine...

08 October 2020 4,943 3 View

Why not there is common reference citation format for all journals?

Why not there is common reference citation format for all journals?

03 October 2020 8,137 3 View

Which force field should be used to simulate protein-protein complex in gromacs?

Hi, I have done a protein-protein docking. Now I want to perform MD in gromacs. I am confused with forcefields. When I tried to findout, everywhere it is written for protein-ligand but I couldn't...

29 September 2020 1,410 3 View

Anyone knows a reliable service who can run my data for me?

Please, if you know such a person or service, kindly email me the contact. I am looking for a reliable service to help me with my data set analyses. Thank you, Y. Essounga: [email protected]

23 September 2020 2,080 3 View

How authentic is this conference, https://www.scientificresearchers.org/icbe-8?fbclid=IwAR2_kI2DGxq7Hlc-Be6gIZKrwgXvjqg2Q5FT2SmIo2yiQecWAcMDpl7GZS0?

Good day, I just to want whether the above conference is a fake or not Regards

17 September 2020 3,912 7 View

Is it possible to run a doped system in CASTEP without creating supercell structure?

I was run a doped system in CASTEP without supercell and it was successfully optimized. But, I am not fully understood when I have to create a supercell. The unit cell I had used contain 28...

06 July 2020 7,657 2 View

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02 March 2021 3,060 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

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22 February 2021 9,932 11 View