We culture B cells in RPMI 1640 (with L-glutamine), 5% human serum, 10 mM HEPES and 1X antibiotics with required stimulants (i.e IL2 or IL4) for our experimets.

Attaching a paper for your reference.

https://www.jimmunol.org/content/jimmunol/early/2016/10/07/jimmunol.1502193.full.pdf

Hope this is helpful.

Please see B-cell culture section from methods part of my paper below...

https://www.nature.com/articles/ncomms7217

Thanks a lot!

You could try B cell culture products from Humimmu.

https://www.humimmu.com/collections/b-cell-culture-and-expansion-products

https://www.humimmu.com/collections/b-cell-culture-and-expansion-products/products/human-b-cell-culture-and-expansion-kit

https://www.humimmu.com/collections/b-cell-culture-and-expansion-products/products/irradiated-3t3-mscd40l-feeder-cells