Can anyone share native PAGE protocol for collagen type I detection?

29 November 2022 1 5K Report

SDS-PAGE details are available for collagen type I and I could get the bands of subunits of the same. I need the protocol for native-PAGE too.

Bimol C Roy

file:///C:/Users/rimol/Downloads/Native%20type%20collagen%20SDS-PAGE.pdf

Badges
Science topic

More Parimala Karthik's questions See All

Citations and h-index ?

How to increase Citations and h-index?

13 June 2024 3,583 1 View

What are the recent research topics in combinatorial design theory related to magic rectangles?

Magic squares/latin squares

01 May 2024 8,098 0 View

How to add Graphene material in sentaurus TCAD ???

where to get the material properties that need to be inserted in the .par file

08 February 2024 7,961 0 View

How does polarity affect welding? Whast is the arc physics (in detail) behind this phenomenon?

I am getting conflicting information from the web. Some say it is one way for arc welding and another way for tig welding and mig welding. I need some clarity as to how arc physics works. There is...

05 February 2024 3,017 2 View

Is there any method available for saponification of Paprika?

Is there any method available to saponify the coloring pigments in Chilli?

09 November 2023 800 0 View

Is there any recommended voltage/current to run a 18% SDS-PAGE gel?

To briefly explain my experiment, I add a chemical probe to the protein lysate, the probe covalently binds to several proteins in the lysate and further the probe is tagged to a fluorophore by...

12 October 2023 5,176 0 View

A typical silicon based transistor's Base-Emitter threshold is 0.7 Volts. What if we dope p type with silicon and n type with Germanium or vice versa?

Diving into to the basic concept of semiconductors, we know the Base-Emitter voltage for a typical silicon based transistor is 0.7 Volts when both p type and n type are doped with silicon. What...

31 July 2023 3,628 1 View

Is there any research papers available on yoga effecting on incretin hormone levels?

how the yoga increasing incretin hormone levels along with mechanisms?

28 May 2023 3,164 3 View

Is there any research papers available on the effect of yoga on epigenetics?

how yoga is affecting the epigenetics and is there any mechanisms available?

19 May 2023 4,114 2 View

How to do optimization using linear programming in Integrated Farming Systems?

Optimization is a statistical technique and done by using linear programming. it is being used in farming systems.

11 April 2023 6,647 5 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

Comparison of Methanol and ethanol treatment on collagen based scaffolds?

Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you

24 July 2024 3,937 0 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What is the cause of this aggregation just bellow 70 kDa mark?

Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...

21 July 2024 5,128 4 View

Fixation Problem with cancer cells?

Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...

19 July 2024 3,906 0 View

Less substrate signals towards the last lanes of the Native PAGE?

I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...

17 July 2024 6,213 4 View

Anybody can assist me in Protein and magnetic beads seperation?

Hi, I hope you are doing well, I am working to remove DNA from protein sample by using silica hydroxode magnetic beads. I used different binding buffers but I din't get my protein band in initial...

02 July 2024 5,502 4 View

How accurate is Image J for SDS bands analysis?

I want to check my protein expression but the sample (target protein) on my SDS PAGE shows a little difference as compared to the control. I measured the band density through IMAGE J but the band...

01 July 2024 1,841 3 View