In particular, my target is Taxus spp. So far, i have tried Sigma kit as well as TriReagent Methods but unsuccessful.
Have seen some literature also but the protocols there seemed a bit complicated.
Have u tried CTAB Method?
Dear Iftekhar
Thanks, but i am still to try it out.. Have you been using the same?
Yes it worked well on apple and Juniper. I am attaching buffer preparation method and the protocol for RNA extraction. It may help u out
Hi Satpal did you try Trizol method.
Hi Abhijit
ya, i did but as mentioned above (TriReagent)...did not work.
currently, am trying Iftekhar's protocol....lets see..
Hi satpal,
It will be better if you try Lithium chloride precipitation along with CTAB method.
Dear all
Thanks for your help. we are now getting the RNA by CTAB + Activated charcoal method.
Best
Satpal Singh
Dear Satpal. Can u share CTAB + Activated charcoal method?
This is the paper
Activated charcoal-mediated RNA extraction
method for Azadirachta indica and plants highly
rich in polyphenolics, polysaccharides and other
complex secondary compounds
Raja Rajakani et al
Rajakani et al. BMC Research Notes 2013, 6:125
http://www.biomedcentral.com/1756-0500/6/125
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