Have u tried CTAB Method?

Dear Iftekhar 

Thanks, but i am still to try it out.. Have you been using the same? 

Yes it worked well on apple and Juniper. I am attaching buffer preparation method and the protocol for RNA extraction. It may help u out

Hi Satpal did you try Trizol method.

Hi Abhijit

ya, i did but as mentioned above (TriReagent)...did not work.

currently, am trying Iftekhar's protocol....lets see..

 Hi satpal,

It will be better if you try Lithium chloride precipitation along with CTAB method.

Dear all

                Thanks for your help. we are now getting the RNA by CTAB + Activated charcoal method.

Best

Satpal Singh

Dear Satpal. Can u share CTAB + Activated charcoal method?

 Dear Iftekhar 

This is the paper 

Activated charcoal-mediated RNA extraction

method for Azadirachta indica and plants highly

rich in polyphenolics, polysaccharides and other

complex secondary compounds

Raja Rajakani et al 

Rajakani et al. BMC Research Notes 2013, 6:125

http://www.biomedcentral.com/1756-0500/6/125

Best 

Satpal Singh