Greetings everyone! Currently, I am engaged in studying fungal diseases related to rice, with a particular focus on Rhizoctonia solani. However, I have encountered challenges when it comes to preserving this particular fungus. Despite my attempts to preserve it using 50% glycerol, I have been unsuccessful in reviving it. In some cases, I have stored isolates in PDA slants, only to find water droplets forming in the tubes. Therefore, I kindly request suggestions on alternative and improved methods for isolating and preserving Rhizoctonia. It is worth noting that I have employed PDA as the medium for isolation purposes.
Thank you.
Storage on sterile grain has been a long-term method for Rhizoctonia solani. See for example Webb et al. 2011. Long term preservation of a collection of Rhizoctonia solani using cryogenic storage. Annals of Applied Biology. 158:297-304. This resource mentions several of the other, similar works using storage on sterile grain. It also references some of the other storage methods, but the colonized sterile grain has been the most reliable we have tried. We particularly like barley as the grain.
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