Hi everyone, I am researching about the potential use of the liquid byproducts of the carbonization process of Gmelina aborea wood residues (i.e. wood vinegar and wood tar), as fungicides against Ceratocystis fimbriata which is causing a vascular wilt in Gmelina arborea trees.
I am conducting a in vitro experiment but I also want to do an in vivo test. I would appreciate recommendations of methods and procedures.
Thanks in advance,
Jair Granados-Chacón
Pot culture experiment should be conducted with sterilized soil. Soil should be sterilized by passing steam air or pouring hot water to remove the already existing pathogens in soil. After complete cooling the known quantity of inoculum already multiplied in sand maize medium to be added to the soil. And then sow the seeds . After establishment of seedlings apply fungicide with required concentration based on your schedule. Disease incidence to be recorded
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