Hi, I need to transfect mice lung fibroblasts with lenti-cre. I bought one from a company but it didn't work at all, efficiency's super low. Can anyone recommend the premade lentil-cre particles that actually work? I'm in US. Many thanks!
We always order ready to use virus from Addgene, and it works very well.
Just check and order the Cre you need.
Vector Builder is great if you want to customize further, or this one:
https://www.cellomicstech.com/product/52/cre-gfp-lentivirus-cmv-puro
Try multiple viral titers for 6-8h.
All the best!
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