Yapei Huang

2 Questions 2 Answers 0 Followers

Questions related from Yapei Huang

Can anyone recommend Ready-to-use lentivirus-CRE-GFP-puro particles?

Hi, I need to transfect mice lung fibroblasts with lenti-cre. I bought one from a company but it didn't work at all, efficiency's super low. Can anyone recommend the premade lentil-cre particles...

04 March 2022 3,527 2 View

Why do I get very low firefly values of wild type plasmid in luciferase assay, but firefly values of empty vector and mutation plasmid are normal?

I'm doing luciferase assay to determine microRNA regulate one target gene expression. The empty vector is pmiRGLO, wild type is pmiRGLO with 3' UTR of the target gene, mutation is vector with seed...

12 February 2019 4,088 3 View