Can anyone recommend me the catalog number of CpG-ODN to stimulate mouse spleen purified B cell to identify IL6 secretion?

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Divaker Choubey

See Invivogen's website below. For mouse it is: - ODN 1585

Link: http://www.invivogen.com/tlr9-agonist?gclid=CjwKEAiAvauyBRDwuYf3qNyXmW4SJACX9-fXKWW1jV6CJoidT0j0GUdemxQx4vfMpFVBFPa64b_lqBoCWOnw_wcB

Koushik Mondal

Thanks, get it

Madhvi Menon

You can use CpG ODN 1826 (CpGB) from Invivogen. This is a TLR9 agonist that activates B cells. CpG ODN 1585 (CpGA) is a major stimulator of IFNalpha production by pDCs. Hope this helps.

Maria Rosa Bono

CpG oligonucleotide ODN 1826 of Invivogen. To use at 2µg/mL. After 24h of activation you obtain about 2.000 pg/ml of IL-6 as detected by CBA on FACS.

Thanks Madhvi and Maria and Dr. Choubey for your valuable suggestion