Chlorella is used to prepare polysaccharides, from which glucose is separated and purified, and I would like to know the details of the separation process.
It is understood that the RM is crude plant material. You may treat it first with absolute alcohol, heat over water bath. In alcohol polysacharides and Glucose are not soluble. The other fractions like Phenolics and Tannins are soluble in alcohol. Then remove out the alcohol by filtration.
To the solids add distilled water of 50 deg. Celsius. and agitate well. Here only glucose goes into the solution. Polysaccharides at this temperature are not soluble in water. Polysaccharides needs hydrolisation at above 70 deg. C for complete suolubilisation in water.
Filter the water and dried under vacuum at 30-35 deg C to get the Glucose.
40 years ago we used Sephadex 25 (I don't even know if they make it anymore) which excluded (via size exclusion) eluant saccharide molecules that had an MW greater than ~1000 amu.
Dear,
the following sites may help you
https://www.creative-proteomics.com/blog/index.php/polysaccharides-isolation-purification-and-analysis/
https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/protein-purification/purification-or-removal-of-glycoproteins-and-polysaccharides
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