Having trouble with qPCR amplification plots. My curve's never seem to reach a plateau. Any ideas?

The parameters of the curve all seem acceptable, typically reactions results are above 0.9852, -3.1 to -3.6 slope, and 90-110% reaction efficiency. The results seem to be what I would expect from...

04 February 2021 1,138 3 View

Do those who are uninsured in the USA receive Covid-19 inpatient treatment?

I am drafting a paper on the number of hospital beds in the different States of the US and find that half of US States have bed provision equivalent to the less developed countries. This leads to...

16 April 2020 2,775 10 View

Is my initial AP FRET set up suitable?

Hi all! I want to use acceptor photobleaching FRET to look at a potential interaction between two proteins of interest. We have a Leica SP8 in my lab that I have used to try and get an idea if...

09 March 2020 2,349 2 View

How to compare two curves using stats?

I am trying to determine if an equation is suitable to model Pressure drop vs fluid velocity. I have experimentally measured pressure drops for a variety of velocities, forming a curve, and I...

06 March 2020 9,517 8 View

What statistic do I use on non-parametric data where I have a response variable and two interacting categorical explanatory variables?

I was wondering whether anyone had any suggestions as to what statistic to use (I use R Studio). I have some muscle cell circumference measurements (response) and I have two explanatory variable...

27 January 2020 5,835 3 View

What are good Hypothalamus Nuclei Markers?

Does anyone know of any good hypothalamic nuclei markers that can be used for immunohistochemistry purposes?

17 June 2019 4,018 3 View

Ways to validate qualitative research?

Using: Husserlian phenomenological qualitative research, eidetic bracketing, semi-structured interviews & Colaizzi data analysis. What are additional ways to validate research given I will be...

05 June 2019 537 6 View

What is the model of computation for this device?

In our research we have been given a device that may be capable of computation. To determine if this is or is not the case we looked for what could be the logical primitive in the physical...

02 March 2019 1,907 1 View

How do I calculate output of solar panels for a given area?

Hi, I'm a little unsure if I have the correct formula. I'm looking to calculate the potential maximum output for Photovoltaic material when I know the surface area. i.e. peak= Wm^2 x total area...

02 March 2019 2,121 4 View

How do I calculate output of solar panels for a given area?

Hi, I'm a little unsure if I have the correct formula. I'm looking to calculate the potential maximum output for Photovoltaic material when I know the surface area. i.e. peak= Wm^2 x total area...

02 March 2019 9,949 4 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Which alginate is best for making gel for wound dressing application?

We are looking into utilizing alginate as a gel coating on an HDPE substrate for drug delivery. I would like to ask the community about their experience with alginate and which commercial sources...

15 February 2021 4,319 4 View

Any natural extract (that is easily available) or any drug combination triple which i can use against gram negative?

I'm working on gram negative i want to use any natural extract (plant extract) or triple drug combination against its biofilm formation.

14 February 2021 1,403 3 View