How to differentiate carbon dots from fluorescent organic molecules ?

How can I confirm the formed product contains carbon dots and not the fluorescent organic molecules. please suggest

30 December 2020 4,284 3 View

How to encrypt image pixels using DNA coding in MATLAB ?

i need a matlab code for the same

10 August 2020 1,042 2 View

Which scale or tool/instrument can be used to assess suicidal desire?

I want to know about scales which can measure suicidal desire, lowered fear of death, increased pain tolerance. Kindly suggest me some authentic scales which can be used...

05 February 2020 1,185 3 View

How do I calculate the superficial gas velocity in a stirred tank reactor?

Air is supplied through a ring sparger which has 6 holes of 1mm each. and suppose the air flow rate is 1 L/min. How can I calculate the superficial gas velocity.

29 January 2019 835 11 View

How can one exactly calculate the volumetric mass transfer coefficient in a stirred tank bioreactor once cells start growing inside the reactor?

I am currently working on hydrodynamics of stirred tank reactors for cell cultures and we are calculating the kLa and I want to know how can we exactly calculate kLa once cells start growing...

27 March 2018 184 46 View

Is there anybody working on the CFD simulation of stirred tank reactors? Please let me know?

I am actually simulating the stirred tank reactor using CFD. I am using the Ansys Fluent to carry out the simulations. I am able to carry out the simulations for the single phase (water). However,...

18 December 2017 6,219 10 View

Which software is best for testing modified networking protocols like DHCP ,ARP etc?

I want to test performance of modified DHCP,ARP etc protocols for that I am need of software .Thanks in advance for your help

05 May 2017 5,138 6 View

What is the best way to construct a pharmacophore model?

If we encounter a situation, where one can construct a pharmacophore model via multiple-complex based as well as via Molecular dynamics based. What is the best way to proceed further and which one...

12 January 2017 8,137 9 View

If we have only two variables, one dependent and one independent, can we estimate the impulse response function?

23 March 2016 6,052 3 View

Phospho-specific TrkB antibodies?

19 November 2015 6,415 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View