Can anyone recommend a reliable method for extracting plasmid DNA from E.coli?

More Zarifah Chowdhury's questions See All

What are the key methods and indicators used in assessing the biodiversity of river ecosystems, and how do these methods account for variations ?

Biodiversity assessment of river ecosystems is crucial for understanding the health and stability of these environments. This question aims to explore the various techniques employed to evaluate...

07 August 2024 4,290 3 View

What is the average energy consumption per gate operation with superconducting qubit?

Could you provide information on the average energy consumption per gate operation when using superconducting qubits, specifically focusing on the microwave power required to implement gates? I...

15 July 2024 954 0 View

What is the most convenient way to pollinate tomatoes?

In terms of labor, cost, and pollination efficiency, what is the most convenient way to pollinate tomatoes grown under controlled environment agriculture facilities, especially in greenhouses?...

18 June 2024 3,825 2 View

Poor CFA factor correlation?

I am currently using the Rosenberg Self-Esteem Scale and the Generalized Self-Efficacy Scale (GSE) to measure the psychological strength of young adults. Finally I have to create psychological...

08 June 2024 1,829 3 View

Piezoresponse Force Microscopy and it’s artefacts?

How can someone remove different artefacts leading to false results due to electrostatic and flexoelectric effects during PFM measurements? during measurement what steps can be taken to nullify...

02 June 2024 8,242 0 View

I need to perform a protein-protein molecular dynamic simulation on GROMACS. Can anyone help me with the complete scripts?

The video tutorial and additional script we found are for the protein-ligand complex. But, in the case of protein-protein, we encounter difficulties. We require help!

28 May 2024 5,500 2 View

What to do when GST- tagged protein is getting precipitated during dialysis?

I purified my recombinant GST-tagged protein in Tris buffer at pH 7.6 (the protein’s PI is 7.11) using a 50 mM reduced glutathione concentration in the elution buffer. However, after dialysis to...

21 May 2024 9,951 2 View

Do anyone have RevMan software for meta-analysis?

I barely need this for meta-analysis in my study... but it cannot be downloaded from the server.

12 May 2024 8,892 3 View

Why digestion like band appear in pcr product?

my gene of interest size is about 4kb. I used hi fidelity taq pol as well as Q5 from NEB but still its happening. my forward primer composed of a 4 adenosine then restriction site followed by 6x...

05 May 2024 4,558 2 View

Which solvents and what ratio should I use for TLC to seperate my azo schiff base and azo aldehyde?

I have synthesized an azo Schiff base using an azo aldehyde and an amine. Now I can not separate my compound in TLC plate. I have used n-hexane and ethyl acetate mixture, acetone, and n-hexane...

24 March 2024 1,099 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View

Igor Oscorbin

A classical alkaline lysis method described by Sambrook would be helpful, as it requires basic reagents: Tris, HCl, EDTA, NaOH, SDS, KAc, AcOH, and can be performed without RNAse.

Michael J. Benedik

in addition to the classic lysis method, the original boiling miniprep also works quite well and requires few reagents as well.

Birnboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513-23. doi: 10.1093/nar/7.6.1513.

Zarifah Chowdhury

Thank you very much for your excellent recommendations

Igor Oscorbin Michael J. Benedik