I used Cre/Loxp system to selectively delete Bmal1 in a specific tissue. I would like to confirm the deletion by labeling my tissue with a specific Bmal1 antibody. Could please recommend the best antibody and protocol to use?
Thank you
Check here: https://www.thermofisher.com/antibody/product/BMAL1-Antibody-Polyclonal/PA1-46118
I have never used such antibodies for routine IHC but I guess AB from Invitrogen are good !
Creative Diagnostics offers antibody for Bmal1 produced in HEK293T cells, and they also provides stable protocol to support your projects.
Hope it can help you.
https://www.creative-diagnostics.com/Anti-ARNTL-MAb-160769-144.htm
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