Hi Robert,

I make protein extracts from Xenopus embryos using the embryos lysis buffer containing 150mM NaCl, 50mM Tris HCl pH7.5, 5mM EDTA, 5mM EGTA and 0.5%NP40. This works really well. You mesh the embryos using a pipette, vortex them hard and incubate on ice for 10min with intermittent vortexing. Post incubation, centrifuge them in a small, table-top, cold centrifuge at max speed for 10mins, and take the supernatant as your lysate.

This should work for you as well. In case your worms are a bit hard, you can add a bit of a stronger detergent and may be sonicate as well. But first I will try the protocol as it is.

Good luck,

Nitin

It is better to use PBC 0.01 M, pH 7.4 and use of Mortar and Pestle for homogenization of the tissue under liquid nitrogen. Then centrifuge the hemogenate at high speed and precipitate other materilas by TCA/acetone method.

I use it for several tissue and found reliable results.