Can anyone recommend a lab for DNA sequencing ?

More Dalveer Singh Somal's questions See All

What is the range of available Potassium(kg/ha) in soil in terms of high, low, medium?

In the case of organic carbon 0.50-0.75 lies in the medium range similarly what is the standard range of potassium in the soil?

30 January 2022 6,059 5 View

How to prepare 1M ammonium acetate buffer and how much amount of glacial acetic acid will be added to it?

Please explain with calculation

13 July 2020 3,357 5 View

How to maintain the ph of soil?

In desert area divesity of plants and animals are decrease due to climatic conditions.some areas are fertile for plantation and animal habitation. due to pollution the ph of soil is change.so...

17 June 2019 5,019 7 View

What is affect of plastic pollution on aquatic diversity?

Plastic pollution in fresh water and marine water has become a global crisis. Plastics pollution has deadly effect on wildlife. Thousands of seabirds and sea turtles, seals and other marine...

10 June 2019 8,014 6 View

How to increase the flora and fauna on earth?

Today is the world environment day so i discuss the very important topic . because the temperature of earth is increased caused by global warming. so the large amount of flora and fauna of earth...

04 June 2019 7,826 13 View

I am looking for Gamification research that explores the relationship between motivation (intrinsic and extrinsic) and employee engagement

I am looking for research about gamification models in organizations. I am focusing on exploring the relationship between motivation and employee engagement to conduct a meta-analysis. I wanted to...

30 July 2017 5,144 3 View

What do you think about environment?

Population is increasing. It’s obvious, if the population increased then the pressures on natural resources increase. Govt and media are busy with other things. But the necessary and main thing is...

01 January 1970 9,555 5 View

How to preserve the biodiversity?

Many reason are responsible to degrade biodiversity like natural and man made. These reasons are the major effect to species of all organisms. This is wide practical unsolved issue.

01 January 1970 7,573 8 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

How should we increase the quality of RNA extraction?

I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...

22 July 2024 155 4 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View

Is there a method that I can measure alcohol dehydrogenase activity using NAD+ in enzyme extraction from plants?

17 July 2024 935 1 View