Can anyone recommend a good secretion signal sequence for protein expression in CHO or HEK cells?

11 November 2017 1 4K Report

Looking to express and purify single-domain antibodies in a mammalian system and would like to have them secreted into the media.

Alessandro Mora

Hi Thomas, I am not an expert on single-domain Ab , but the Azurocidin seems to be a safe choice for conventional MAbs. Look up Kober 2013 for references.

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