Can anyone recommend a good marker for the Golgi Apparatus?

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Ron Reiserer

Stephen Rogers has a great post on IHCWorld that you may find helpful. http://www.ihcworld.com/_protocols/immunofluorescence/cellular_structure.htm

César Osorio-Fuentealba

Hi! Maria Grazia I recommend you this antibody from Cell Signaling http://www.cellsignal.com/products/primary-antibodies/golgin-97-d8p2k-rabbit-mab/13192

Jordan R. Yaron

Fluorescently tagged wheat germ agglutinin (WGA) will stain the Golgi and plasma membrane (https://biotium.com/product/wheat-germ-agglutinin-cf488a-conjugate/). That will be your cheapest option and the signal of the CF dyes from Biotium is extremely nice.

My lab also uses GM130 and GODZ antibodies for staining Golgi.

Tilo Wolf Eichler

Abcam has some staining chemicals called CytoPainter which are working nicely in both life and fixed cells. One is a good Golgi marker with green fluorescence. I use them frequently in different cell types and they work nicely.

Good luck with your immunofluorescence experiments!

Maria Grazia Del Giudice

Thank you all for Your good advices!

After a bit of studying and thinking about how I would build my experiment, I concluded that a "Golgi tracker" would be better than an antibody for my experiments.

I checked about WGA as Jordan Robin Yaron ·suggests but I need to not co-stain the plasma membrane. Thermo Fisher Alexa conjugated lectins or the abcam CytoPainter suggested by Tilo Wolf Eichler, may be the best choice for me but I couldn't find any reference about using them in immunofluorescence protocols.

Have you ever tried??

But I actually read in the (very interesting) post suggested by Ron Reiserer: "it may be wise to perform the lectin staining after staining with secondaries as IgGs are, themselves, glycosylated and simultaneous incubation my induce formation of antibody aggregates".

Hi Maria! We just published an article where we used the Golgi marker. On page 16 the protocol is described. Here you find the reference:

http://www.mdpi.com/1422-0067/16/9/21658

The protocol following the dye is very good and detailed and you can follow it straight forward. Please, don't hesitate to contact me if you need help or advise! Good luck :)