We frequently use the I-125 CORT RIA kit from MP biomedicals for mice and rats, but I have never measure ACTH from rat plasma before. I am wondering if anyone can recommend a good validated RIA kit for rat ACTH. Thanks.
You could contact to Dr. Parlow group at UCLA. (NATIONAL HORMONE and PEPTIDE PROGRAM). They developed kits for a lot od hormones (human, rats, mice, guinea pigs, etc.). We used their rat PRL RIA kit, and antibodies for all pituitary hormones for immunofluorescence.