Can anyone recommend a [good] primary antibody for glucocorticoid receptor? We previously used H-300 or M-20 from Santa Cruz Biotechnology. We plan to use the antibody for IHC in rodent brain tissue.
Have a look at this nice paper from Onno Meijer a few years ago:
Sarabdjitsingh RA, Meijer OC, de Kloet ER
Specificity of glucocorticoid receptor primary antibodies for analysis of receptor localization patterns in cultured cells and rat hippocampus. Brain Res. 2010 May 17;1331:1-11.
https://doi.org/10.1016/j.brainres.2010.03.052
It looks like the mouse monoclonal BuGR2 (aa 407–423 of rat GR) from Abcam might be OK.