Is there any website or online source to draw the architecture of the neural network ?

I have the details of the input, output, number of hidden nodes and number of hidden layers data. How can I draw the architecture of the neural network for my condition.

21 October 2020 6,648 1 View

How to model geosynthetics in stone column encasement (abaqus)?

I am using abaqus to model geosynthetic encased stone column (wished in-place). I am new to abaqus so how should I model geosynthetics in 3D and 2D (solid or shell or wire ) and can I get an idea...

17 September 2020 5,165 3 View

Is it possible to get the probable lattice structure of crystalline powder sample using miller indices (h, k, l values) and XRD data?

Hello everyone, presently, I am working on crystalline properties of some polymers with respect to their individual treatments using X-Ray Diffraction (XRD). Is there any software which can give...

08 September 2020 1,223 5 View

How to increase the yield of extracellular DNA in extraction process from water samples?

I am using 1% CTAB for extracellular DNA extraction, followed by isopropanol precipitation, phenol chloroform extraction, ethanol precipitation and wash. How can I increase my yield?

02 March 2020 7,947 3 View

How to fix the Open cv module error?

I am working on image segmentation using python open cv package but couldn't able to work with it because of error indicating "ModuleNotFoundError: No module named 'cv2'" and the same error is...

02 February 2020 928 4 View

Is there a good Piezo1 antibody available commercially?

Can anyone suggest an antibody for Piezo1 that has been used in good publications for immunoflourescence stainings?

10 October 2019 9,995 1 View

How can I use sonication to get lysosomal membrane protein?

I am trying to standardise lysosomal enzymes assay. Since the enzyme is active inside the lysosome and is partially embedded in the lysosomal membrane, lysis of cells with lysosomal membrane is...

10 February 2019 7,653 2 View

I have completed my bachelor's in Electrical Engineering and I am looking forward to start learning more about smart grid .From where should I start.?

I want to learn about smart grid and simulating a smart grid network and also the data analytics used in smart grid network .

23 May 2018 6,165 3 View

Is fluorescence from boiled residues of sugars/oils/acids due to carbon quantum dots or some other fluorescent compounds?

I tried making carbon quantum dots by microwaving aqueous solutions of citric acid as a simple science outreach activity. I followed this literature precedent ( Milosavljevic, V., et al. J...

23 May 2018 950 3 View

Does sequential treatment for monocytes differentiation in to macrophages (in vitro) affect the generation of cytokine pattern after stimulation?

I would like to know if in vitro derived macrophages (from monocytic cell line) by the treatment of 2 stimulants/chemicals (for differentiation) subsequently would affect the cytokine profile...

22 March 2018 5,029 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View