PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Please let me know how to remove mycoplasma contamination from cell line?

I am facing a problem in maintaining the cell line of HepG2 and HepG2.2.15. I have used plasmocin Tm treatment (Invivo gen) Cat.#ant-mpt Lot #MPT-41-01 for more than a month. I have also used...

21 February 2021 6,046 4 View

Algorithms to filter corrective max and min from a set of data?

I've got this 5 year Direct Normal Irradiance data I downloaded from https://re.jrc.ec.europa.eu/. I ordered this by year and by every 24 hours because I need the DNI curve by max in summer, min...

17 February 2021 2,777 5 View

Western blot protein of interest falling at a lower weight than expected?

My protein of interest is DAGLa (115 kDa). We received the antibody for this from another researcher who reliably shows bands at this weight. We do see a light banding at this weight, but the...

11 February 2021 6,009 3 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

Is there variability in dorsal glow spots in Lamprohiza mulsantii also in thoracic segments ?

I decided to edit this question to show the right/ correct things and erase those clearly wrong in order to avoid confusion....

03 October 2020 5,889 3 View

Is simulation technology has any role in laparoscopic surgical training ?

Minimally invasive surgery technique has taken the central role in modern surgery specially in abdominal surgery. So, every surgeons need to get trained into the field of minimally invasive...

25 May 2020 6,412 9 View

How sure are you, that after TUR-B for NMIBC, to have enough muscle tissue for correct histopathological examination?

Deep resection for correct histopathological analysis in TUR-B can be challenging for the surgeon with a low to mid-experience level. This leads to inadequate analysis, possibly postponing radical...

11 April 2020 5,713 3 View

What is this structure in the laparoscopic image?

This is an operative image during laparoscopic surgery for hiatal hernia. What is the structure in this image referred to it by an arrow? Is it thoracic duct or another structure?

15 February 2020 7,770 14 View

What is this structure in the laparoscopic image?

This is an operative image during laparoscopic surgery for hiatal hernia. What is the structure in this image referred to it by an arrow? Is it thoracic duct or another structure?

01 February 2020 4,611 11 View

Looking for the cause of death in myocardial infarcted mice?

10 November 2019 527 3 View

What is the most reliable compression model (tool) to be used for mice thoracic SCI?

being working on a preclinical research studying spinal cord injury ..

04 May 2019 1,369 1 View

What is the most reliable compression model (tool) to be used for mice thoracic SCI?

being working on a preclinical research studying spinal cord injury ..

02 May 2019 6,433 1 View

Im doing verhoeff van gieson staining, but i could not stain elastic fibers black. nucleus stained black though. any suggestions?

verhoeff: tried 5% alcoholic hematoxylin, 10% ferric and weigerts iodine ( 10ml:4ml:4ml) van giesson: 1% acid fuchsin with saturated picric acid also tried modified vvg by garvey et al, 1991....

03 April 2019 3,868 1 View

Does anyone have an excel/SAS/STATA/SPSS file to calculate STS score for isolated aortic valve replacement?

Also, if you have recently calculated coefficients or weights for the variables, I can create an algorithm.

09 October 2018 5,365 0 View