Hi,

The plasmid sequence:

http://www.ncbi.nlm.nih.gov/nuccore/122703336

A reference article:

http://www.bunken.org/jspcmb/journal/pb26_3/26_275.pdf

The free software to create and annotate your plasmid map:

http://www.snapgene.com/

Cheers,

Ruben

The Snapgene website says we have to buy it (licensing; not free), but with a short period of time of Free trail.

Hi, that's the fully functional version you are talking about. The snapgene viewer is a limited one, but it works pretty well. There are other free plasmid makers like plasmaDNA, plasmapper, Ugene, that you may wish to try, although, that said, I recommend the abovementioned for simplicity and flexibility :)

Cheers,

Ruben

Ruben, thanks for the info.

Hello Banaras,

I wouldn't post a link to a crack here, I'd suggest you delete it. Otherwise I'd feel inclined to report it to Researchgate.

There are tons of alternative free software packages that can be used in place of Snapgene like:

- genomecompiler

- benchling

- Ugene

Best,

Ruben