How to Study a proton NMR of Graphene prepared by Electrochemical Exfoliation technique?

I performed a Proton NMR of graphene powder prepared by electrochemical exfoliation technique. It shows some peaks. But I can't understand it.

21 February 2021 8,947 3 View

Can wavelet packet sub-bands be stacked together to form an image?

I have a 1D signal and I have done wavelet packet decomposition on it which is giving several sub-bands. Can I stack these sub-bands (one below other) to form a 2D matrix and hence an image...

27 November 2020 1,269 5 View

Why the IT services companies (based in India) are mainly patenting only at the USPTO?

Please suggest some papers/reports on the changes in the policy with respect to patenting of computer software in India. Does it have any implications as to why the IT services companies (mostly...

12 November 2020 8,351 1 View

Loop for summation in MATLAB ?

for different values of X, what will be the loop or tool in MATLAB for the below problem summation (U(n-L)(X)*(K(L)-K(L+1)), 1, n-1), where U is the function of X for different time (n-L), and...

18 April 2020 7,047 11 View

Why does HeLa transfected with connexins grow at a much slower rate and form colonies ?

I have two cell lines, one is the wild-type HeLa Cell Line (wt-HeLa), and another is a virally transfected with Connexin-43 HeLa Cell Line (HeLa-43). I have grown both the cultures overnight,...

01 March 2020 8,106 3 View

Is Diethylamine detectable in UV/Vis spectrometer?

I tried to analyse diethylamine in hplc with UV detector but it was not giving satisfactory results. Will using UV/Vis spectrometer in case of diethylamine will be usefell?

27 February 2020 8,686 3 View

Does the aqueous extract of Neem bark have same constituents and composition of any other organic solvent extract of Neem Bark ?

I am trying to do an experiment where I examining the effect of Neem Bark Extract on cells. I have two sources for the extract. a) Self-made Methanolic Neem Bark Extract and b) Aqueous Neem Bark...

07 February 2020 3,076 3 View

I performed a DSC experiment in Alumina (Al2O3) crucible. The alloy appears to have reacted with crucible itself. What can be the reasons for this?

I am working on Zr based metallic glass which reacted with the Alumina crucible in which I was performing the DSC experiment. Kindly suggest possible reasons for this anamoly.

23 January 2020 8,039 2 View

How I can remove metals from an enzyme experimentally? Please provide detailed protocol, if possible step by step?

I previously tried some protocol to remove metal from enzyme to make apoenzyme, but it did not work. Questions in this regard: 1. My apoenzyme gave 20% activity but in many literary sources...

26 December 2019 8,079 3 View

How can I quantify the effect of electronic configuration of participating elements in its role in increasing the entropy of High Entropy Alloys?

I wish to incorporate the electronic interaction entropy change in the calculation of total entropy of a high entropy alloy. Please suggest ways to quantify it in case of any arbitrary equiatomic...

24 June 2019 3,482 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View