Anurag Dhiman

6 Questions 2 Answers 0 Followers

Questions related from Anurag Dhiman

Can anyone help me with clonnig of HSP90.1 protein of Arabidopsis thaliana?

I am trying to clone HSP90.1 cDNA. For that, I have extracted RNA of Arabidopsis from the leaf. From RNA, cDNA is formed and used for PCR using primer. But I am not getting anything. I have tried...

25 April 2022 6,342 3 View

What are the different controls needed for inhibition assay and how to calculate percentage inhibition for IC50 calculation in enzymatic assay?

I am performing enzymatic inhibition assay by using flouregenic peptide containg AMC modification. I am trying to calculate percentage inhibition. But I don't know what are different controls...

03 March 2022 3,807 0 View

How to find Vmax and km from enzyme activity assay?

I have performed enzyme (protease) activity assay using flouregenic peptide (containing AMC at c terminal) as substrate at different concentrations of substrate. I have RFU values with respect to...

22 February 2022 3,258 3 View

Can anyone provide me protocol for preperation of BL21 competent cells using chemical method?

I need BL21 competent cells. But i don't have protocol for preparing BL21 competent cells. Kindly help me with this. provide if any standardised protocol anyone have or used?

29 December 2021 738 1 View

can Histidine tag interfere with protein activity?

I have purified a recombinant protein and I am not getting any protein activity in activity assay. Protein contains histidine tag. Is there possibility that his tag is interfering with protein...

14 December 2021 8,153 4 View

Can coloured compounds interfere in flourescence substrate based inhibition assay?

I am performing inhibition assay of a protein by using substrate containing flourecent group modification. The inhibitory substances i am using are coloured molecules and extracts. In most of them...

01 January 1970 9,454 1 View