We don't have option of Synovectomy so we have to do HFLS culture from Synovial Fluid. I am trying to culture FLS from synovial fluid of the patients with RA but as this process of drawing SF from patients are taking place in open enviroment, contamination is taking place every time .How can I avoid it ? Secondly I want to know what is the chance of getting the fibroblast like properties of this synoviocytes cultured from Synovial fluid. Is this a good target to study the inflammatory pathway?