Shilpa Sengupta

4 Questions 3 Answers 0 Followers

Questions related from Shilpa Sengupta

What are the roles of memory B cells toward antibody response in Visceral leishamniasis ?

We are trying to analyse the role of memory B cells in in patients with Visceral leishmaniasis by flow cytometry using the marker CD27 and IgD . We are getting increased frequency of memory b...

18 May 2019 3,575 3 View

Can anyone please share the protocol of culturing Human fibroblast like synoviocytes from Synovial fluid of the Patients with Rheumatoid Arthritis ?

We don't have option of Synovectomy so we have to do HFLS culture from Synovial Fluid. I am trying to culture FLS from synovial fluid of the patients with RA but as this process of drawing SF...

09 June 2018 9,650 2 View

How the Human B regulatory cell subtype B10 and Br1 differ from each other?

I want to have full characterization of Human B regulatory cells in peripheral blood of patients with Visceral Leishmaniasis and PKDL But I don't have antibody for B10 cells (CD27 CD38 CD24). So...

17 February 2018 4,922 3 View

Does cytokines have any role in causing metabolic switch in immune cells like lymphocytes in autoimmune disorder like Rheumatoid arthritis?

I am currently working on metabolic reprogramming of immune cells in autoimmune disorder rheumatoid arthritis . basically I want to see what is the metabolic State of this cell in blood during...

03 February 2018 9,632 2 View