What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

Any recommendations for tetR antibody for IHC?

I am planning an experiment where I've to detect cells which are expressing TetR, my vector is so big that I can't have a reporter in it. I was thinking if I can detect the expression of TetR...

24 September 2020 1,041 4 View

How do I reduce the size of a file in Comsol simulations?

I am working with RF module in comsol and doing parametric sweeps in frequency domain. The data generated is huge and taking a lot of space on my disc. The fine meshing that I need for my problem...

22 July 2020 4,374 6 View

How to get the antenna to resonate at the required frequency with the ideal magnitude in the s-parameter graph?

I have designed a rectangular patch antenna for a frequency of 2.4Ghz using Rogers 5880 as my substrate material with a thickness of 1.6mm. But, my s-parameter graph does not show a graph with the...

03 October 2019 6,543 4 View

Best reagent for Colorimetric detection of chloride ions?

I want to find an assay for colorimetric method of detection of chloride ions from blood or body fluids. The main issue is I cannot use mercury or mercury derived chemicals in my assay. Can...

04 February 2019 8,093 3 View

How to grow N.gonnorhea?

02 August 2016 1,264 1 View

I tried extracting plasmid DNA by Brimbroim Dolly method. After running on agarose gel I am getting mutiple bands of different molecular weights.why?

Are all the bands plasmids itself?or is it degraded plasmid?

30 July 2016 7,069 11 View

What can be the possible outgroup for Ryphilla sp (crab, leucosidae) while drawing phylogenetic tree with Co1 gene??

Which all organisms can be chosen as best outgroups for Ryphilla crabs in the phylogenetic tree

30 July 2016 9,087 1 View

Can you provide any method for APEO analysis on HPLC?

bufffer of what pH can we use as mobile phase?

01 March 2016 8,861 1 View

What is the difference in FWHM from Guassian and Lorent fit used in Scherrer formulae?

I have calculated the crystallite size of NiO by using the well known Scherrer formulae. I fit the XRD peaks and determined the FWHM both by Gaussian and Lorentz fit however the crystallite size...

14 June 2015 770 27 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View