Can anyone please send me the procedure for plasmid extraction from Vibrio species?

More Reshma Silvester's questions See All

What are the methods for analyzing trihalomethanes in water other than gas chromatography and liquid chromatography?

for monitoring disinfection byproducts in drinking water

12 October 2023 8,613 3 View

I am doing base hydrolysis of ester with LiOH and THF:H2O( 1:1) but after hydrolysis in acid NMR I am getting 4 proton extra peak?

4 protons peak may be CH2 near a heteroatom .

13 July 2023 1,075 1 View

How to reduce air bubble formation in film?

The film forming solution (FFS) is stirred overnight and it is oven dried at 40°C. Please give me suggestions to reduce the air bubble.

09 July 2023 7,857 4 View

Does anyone know how to prevent protein coeluting with other impurities in size exclusion Column?

I have been trying to purify a transcription factor. Protein has MBP and His Tag. I use His TRAP as my first purification step and then after I do size exclusion, but my protein (83 KDa) elutes...

23 April 2023 9,182 5 View

How to recover the Pd(PPh3)4 catalyst, already become dark brown?

How to store this catalyst?

06 March 2023 9,026 1 View

How do I determine doping concentration of a new element in Barium Titanate structure if I want to produce a new system?

I want to produce a new system of Barium Titanate and characterize it for high dielectric constant values at microwave frequencies.

02 February 2023 4,203 8 View

How to find Yaw joint angle of differential drive robot ?

I'm designing leader follower feedback controller for crawler robots .Here rear wheel is connected to front via a joint so that it can follow front when it turns ,for both the module(front...

20 December 2021 1,203 3 View

How can I quantify the amount of cyphenothrin in a solution using titration?

I have tried hydrolysis with KOH then titrating the Cyanide Ions liberated with Silver Nitrating but I am not getting results. Anyone with a better method please help. I'll be very grateful since...

12 September 2021 5,689 3 View

Multivesicular liposomes formulation?

multivesicular liposomes are prepared by double emulsification method. Can someone tell how to evaporate organic solvent from multivesicular liposomes preparation without emulsion cracking?

04 March 2021 5,166 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,871 6 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View